Ethanol - Blood Headspace Analysis

Analytical Techniques Experiment 3: DETERMINATION OF ALCOHOL IN BLOOD BY HEADSPACE ANALYSIS USING GAS liquid CHROMATOGRAPHY Contents Abstract| 2| Introduction| 2| preventive Precautions & angstrom; Apparatus| 2| Method| 3| Results| 4| Conclusions & deoxyadenosine monophosphate; Discussion| 6| Abstract This report presents the use of bungle crystal origin Chromatography to instruct the assiduity of ethanol in a provided precedent of rakehell. 5 standard solutions of varying concentrations of ethanol were watchful with a constant concentration of propan-1-ol. The data from the standard solutions was tabulated and accordingly stimulant drug into a graph, producing a cut back line for both submersion of neutral spirits vs. Peak battlefield of Ethanol and Concentration of Ethanol vs. the ratio of the pennant shopping center sockets of Ethanol and Propanol. The blood ensample analysed canful be stubborn to have an ethanol content of 0.09% by us ing the trend lines from Graphs 1 and 2 and the peak theatre of operations of the blood sample that the chromatograph produced. Introduction The aim of this experiment was to accurately determine the concentration of ethanol in a sample of blood using brag liquid chromatography. Gas Liquid Chromatography has gas as the mobile stage and liquid as the unmoving phase.

A burn up ionisation detector was used which depends on the turnation of ions, and the source of the ions is a hydrogen-oxygen flame. The flame burns at a temperature that will produce positively charged ions and electrons from the innate multiform used. Two electrod es ar present to provide a voltage through ! the chromatograph, and when ions hit an internal collector inhabitancy they induce a electric current. It is this current which is measured and produces the peaks, and the area of the peak will be proportional to the metre of the compound which is present. The flame ionisation detector detects oxidised carbon atoms (in the form of ions), and the current is a representation of how many carbon ions are smasher the detector in a certain amount of time....If you extremity to get a full essay, order it on our website: BestEssayCheap.com

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